software graphpad prism version 10.2.2  (GraphPad Software Inc)

Journal: Journal of Bacteriology

Article Title: Borrelia burgdorferi loses essential genetic elements and cell proliferative potential during stationary phase in culture but not in the tick vector

doi: 10.1128/jb.00457-24

Figure Lengend Snippet: B. burgdorferi cells in Ixodes scapularis nymphs starved for 14 months remain proliferative. (A) Schematic of the experiment. Four to 8 weeks old female mice were infected with B. burgdorferi strain CJW_Bb474 by needle inoculation. Larval I. scapularis ticks became colonized with this strain during feeding on the infected mice. The fed larvae were allowed to molt into nymphs. These nymphs were kept unfed at room temperature for the indicated durations before their spirochete loads were determined by plating. The mouse and tick cartoons are adapted from a previous publication . (B) B. burgdorferi burdens in unfed nymphs that were generated as described in ( A ). The B. burgdorferi burdens were measured 1 month or 14 months after molt by semisolid BSK-agarose plating and are expressed as log 10 (CFU/tick). Shown are means ± standard deviations for five or six individual ticks. The individual values are indicated by black dots. The unpaired t -test with Welch’s correction on the log 10 -transformed data gave a P -value of 0.036. The 1-month and 14-month nymphs came from the same cohort. The 1-month data, which were previously published , are shown here for comparison.

Article Snippet: For , the linear, normal scale CFU data were converted to log-normal data via a log 10 transformation, then were compared using an unpaired t -test with Welch’s correction in GraphPad Prism version 10.2.2 software.

Techniques: Infection, Generated, Transformation Assay, Comparison